You can check my updated publication list here. You can read my Ph.D. thesis (May 2021) here.


Huseyin Tas, Adam Amara, Miguel E Cueva, Nadine Bongaerts, Alicia CalvoÔÇÉVillama├▒├ín, Samir Hamadache, Konstantinos Vavitsas. Are synthetic biology standards applicable in everyday research practice?. In Microbial biotechnology.

Alicia Calvo-Villama├▒├ín#, J├ęrome Wong Ng#, R├ęmi Planel, Herv├ę M├ęnager, Arthur Chen, Lun Cui#, David Bikard (# equal contribution).On-target activity predictions enable improved CRISPRÔÇôdCas9 screens in bacteria. In Nucleic Acids Research.

Alicia Calvo-Villamañán, Aude Bernheim, David Bikard.Methods for the Analysis and Characterization of Defense Mechanisms Against Horizontal Gene Transfer: CRISPR Systems. In Methods in Molecular Biology, Horizontal Gene Transfer.


Aude Bernheim, Alicia Calvo-Villamañán, Clovis Basier, Lun Cui, Eduardo PC Rocha, Marie Touchon, David Bikard. Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria. In Nature Communications.